Human IDS HEK293 Cell Lysate

Name: Human IDS HEK293 Cell Lysate
SKU: CLOE-1507
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1507 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1507
Introduction	This cell lysate over-expresses C-terminal polyhistidine tagged human IDS precursor (NP_000193.1) (Met 1-Pro 550). Purification of target protein (s) from this cell lysate has been verified.
Target Gene	IDS
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	IDS iduronate 2-sulfatase [ Homo sapiens ]
Gene Symbol	IDS
Synonyms	IDS; iduronate 2-sulfatase; SIDS; Hunter syndrome; idursulfase; iduronate 2-sulfatase 14 kDa chain; iduronate 2-sulfatase 42 kDa chain; alpha-L-iduronate sulfate sulfatase; MPS2;
Gene ID	3423
Uni Prot ID	P22304
m RNA Refseq	BC006170
Chromosome Location	Xq27.3-q28
Function	catalytic activity; hydrolase activity; iduronate-2-sulfatase activity; metal ion binding; sulfuric ester hydrolase activity;
Pathway	Dermatan sulfate degradation, organism-specific biosystem; Dermatan sulfate degradation, conserved biosystem; Glycosaminoglycan degradation, organism-specific biosystem; Glycosaminoglycan degradation, conserved biosystem; Heparan sulfate degradation, organism-specific biosystem; Heparan sulfate degradation, conserved biosystem; Lysosome, organism-specific biosystem;
MIM	300823

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