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Human HERC5 Knockout Cell Line-A549

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT2235

Target Gene :   HERC5 Host Cell :   A549

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RT2235
Description This cell is a stable cell line with a homozygous knockout of human HERC5 using CRISPR/Cas9.
Target Gene HERC5
Host Cell A549
Host Cell Species Homo sapiens (Human)
Size >1x106 cells/vial
Validation Sequencing
Storage Liquid nirtogen
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name HERC5 HECT and RLD domain containing E3 ubiquitin protein ligase 5 [ Homo sapiens ]
Gene Symbol HERC5
Synonyms CEB1; CEBP1
Gene ID 51191
Uni Prot ID B4DXV3
m RNA Refseq NM_016323.3
Protein Refseq NP_057407.2
Chromosome Location 4q22.1
Function ISG15 ligase activity; protein binding; ubiquitin-protein ligase activity;
Pathway Antiviral mechanism by IFN-stimulated genes, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; ISG15 antiviral mechanism, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; Interferon Signaling, organism-specific biosystem; Negative regulators of RIG-I/MDA5 signaling, organism-specific biosystem;
MIM 608242
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Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
Reliable service

In the process of cell culture, the cell growth was affected by the non-standard culture conditions. We received patient guidance and help after consultation. Like it!

United States

High efficiency

Using prefabricated CRISPR knockout cell lines and lysates helped me save months of time. So I'm considering buying other cell lines here as well.

United Kingdom

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