Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-1529 Host Cell : HEK293
| Cat. No. | CLOE-1529 |
| Introduction | This cell lysate over-expresses C-terminal polyhistidine tagged human FN1 (CAD91166) (Ser 607-Pro 1265). Purification of target protein (s) from this cell lysate has been verified. |
| Target Gene | FN1 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | FN1 fibronectin 1 [ Homo sapiens ] |
| Gene Symbol | FN1 |
| Synonyms | FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2 |
| Gene Description | fibronectin 1 |
| GeneID | 2335 |
| Uni ProtID | P02751 |
| mRNA Refseq | NM_212482.1 |
| Protein Refseq | NP_997647.1 |
| Chromosome Location | 2q34 |
| Function | collagen binding; extracellular matrix structural constituent; heparin binding; protein binding; |
| Pathway | Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; Bacterial invasion of epithelial cells, organism-specific biosystem; Bacterial invasion of epithelial cells, conserved biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem; ECM-receptor interaction, organism-specific biosystem; |
| MIM | 135600 |
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