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Human FANCI Knockout Cell Line-Hela

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT2396

Target Gene :   FANCI Host Cell :   HeLa

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RT2396
Description This cell is a stable cell line with a homozygous knockout of human FANCI using CRISPR/Cas9.
Target Gene FANCI
Host Cell HeLa
Host Cell Species Homo sapiens (Human)
Size >1x106 cells/vial
Validation Sequencing
Storage Liquid nirtogen
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:4-1:6.
Freeze Medium Complete medium supplemented with 10% (v/v) DMSO
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name FANCI Fanconi anemia, complementation group I [ Homo sapiens ]
Gene Symbol FANCI
Synonyms KIAA1794
Gene ID 55215
Uni Prot ID Q9NVI1
m RNA Refseq NM_001113378.1
Protein Refseq NP_001106849.1
Chromosome Location 15q26.1
Function DNA binding; protein binding;
Pathway DNA Repair, organism-specific biosystem; Fanconi Anemia pathway, organism-specific biosystem; Fanconi anemia pathway, organism-specific biosystem; Fanconi anemia pathway, conserved biosystem; Regulation of the Fanconi anemia pathway, organism-specific biosystem;
MIM 611360
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Q & A

Customer Reviews

Customer Q&As
What is the recommended growth medium? Does it require antibiotic selection?

A: DMEM supplemented with 10% fetal bovine serum. <br> It is not required to add the selection antibiotics when culturing the KO cells.

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
High efficiency and convenience

The editing process uses a dual gRNA system with an efficiency of more than 95%! It's cool!

United States

Reliable service

The team is available to assist me with any questions about CRISPR knockout cell lines. I feel very good!

United States

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