Human EPO HEK293 Cell Lysate

Name: Human EPO HEK293 Cell Lysate
SKU: CLOE-1581
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1581 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1581
Introduction	This cell lysate over-expresses the human EPO (NP_000790.2) (Met1-Arg193) fused with the Fc region of human IgG1 at the C-terminus. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	EPO
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	EPO erythropoietin [ Homo sapiens ]
Gene Symbol	EPO
Synonyms	EP; MVCD2
Gene Description	erythropoietin
Gene ID	2056
Uni Prot ID	G9JKG7
m RNA Refseq	NM_000799.2
Protein Refseq	NP_000790.2
Chromosome Location	7q22
Function	erythropoietin receptor binding; eukaryotic cell surface binding; hormone activity; protein binding; protein kinase activator activity;
Pathway	Cellular response to hypoxia, organism-specific biosystem; Cellular responses to stress, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; EPO Receptor Signaling, organism-specific biosystem; EPO signaling pathway, organism-specific biosystem; HIF-1 signaling pathway, organism-specific biosystem;
MIM	133170

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