Human EGF HEK293 Cell Lysate

Name: Human EGF HEK293 Cell Lysate
SKU: CLOE-1308
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1308 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1308
Introduction	This cell lysate over-expresses the human EGF (NP_001954.2) (Asn 971-Arg 1023) fused with the Fc region of human IgG1 at the N-terminus. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	EGF
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	EGF epidermal growth factor [ Homo sapiens ]
Gene Symbol	EGF
Synonyms	EGF; epidermal growth factor; epidermal growth factor (beta urogastrone); pro-epidermal growth factor; beta-urogastrone; URG; HOMG4;
GeneID	1950
Uni ProtID	P01133
mRNA Refseq	BC113461
Chromosome Location	4q25
Function	calcium ion binding; epidermal growth factor receptor binding; growth factor activity; protein binding; transmembrane receptor protein tyrosine kinase activator activity;
Pathway	Arf6 signaling events, organism-specific biosystem; Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Ceramide signaling pathway, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Delta-Notch Signaling Pathway, organism-specific biosystem;
MIM	131530

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