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Human DUSP10 adenoviral particles

Human DUSP10 adenoviral particles

Cat.No. :  AD00153Z

Titer: ≥1x10^10 IFU/mL / ≥1x10^11 IFU/mL / ≥1x10^11 VP/mL / ≥1x10^12 VP/mL Size: 100 ul/500 ul/1 mL

Storage:  -80℃ Shipping:  Frozen on dry ice

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Adenovirus Particle Information

Quality Control

Gene Informationn

Cat. No. AD00153Z
Target Gene DUSP10
Product Type Adenoviral particle
Insert DUSP10
Titer Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc.
Size Varies lot by lot, for example, 250 ul, 500 ul, 1 mL etc.
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Frozen on dry ice
Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots.
Endotoxin Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance.
Sterility Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination.
Ad5 E1 Detection All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination.
RCA Assays Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources.
PFU Titering All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells.
Gene Name
Gene Symbol
Synonyms
Gene ID
UniProt ID
mRNA Refseq
Chromosome Location
Function
Pathway
MIM
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The dual-specificity phosphatase 10 (DUSP10) gene, also known as mitogen-activated protein kinase phosphatase 5 (MKP-5), encodes a member of the dual-specificity phosphatase (DUSP) family. These enzymes play a key role in cellular signaling by dephosphorylating tyrosine and serine/threonine residues on target proteins, especially mitogen-activated protein kinases (MAPKs). DUSP10 specifically inhibits MAPKs such as p38 and JNK, which play key roles in stress responses, inflammation, apoptosis, and immune regulation. By regulating MAPK activity, DUSP10 helps maintain cellular homeostasis and participates in various physiological and pathological processes, including metabolic disorders, cardiovascular diseases, and cancer. Its expression is tightly regulated and is often induced by stressors such as oxidative stress or cytokines. Human DUSP10 adenoviral particles are recombinant viral vectors designed to deliver the DUSP10 gene to target cells for functional studies or therapeutic applications. Adenoviral systems are favored for their high transduction efficiency, ability to infect both dividing and non-dividing cells, and robust transgene expression. The particles are replication-defective, which prevents uncontrolled viral spread, ensuring safety. Researchers use DUSP10 adenoviral particles to study the role of this gene in MAPK signaling, immune regulation, or disease mechanisms. The particles are produced through cell packaging, purification, and titration to ensure consistent quality.
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Customer Reviews
Great for In Vivo Studies

Used these adenoviral particles in mouse models, and the infection rate was impressive. Minimal immune response and strong target gene expression. Perfect for translational research!

Germany

03/30/2021

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