Human CTSA HEK293 Cell Lysate

Name: Human CTSA HEK293 Cell Lysate
SKU: CLOE-1381
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1381 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1381
Introduction	This cell lysate over-expresses the human CTSA (NP_001121167.1) (Met 1-Tyr 480) fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	CTSA
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	CTSA cathepsin A [ Homo sapiens ]
Gene Symbol	CTSA
Synonyms	GSL; GLB2; NGBE; PPCA; PPGB
Gene Description	cathepsin A
Gene ID	5476
Uni Prot ID	P10619
m RNA Refseq	NM_000308.2
Protein Refseq	NP_000299.2
Chromosome Location	20q13.1
Function	carboxypeptidase activity; enzyme activator activity; serine-type carboxypeptidase activity;
Pathway	Adaptive Immune System, organism-specific biosystem; Glycosphingolipid metabolism, organism-specific biosystem; Immune System, organism-specific biosystem; Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; MHC class II antigen presentation, organism-specific biosystem; Metabolism, organism-specific biosystem;
MIM	613111

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