Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-1370 Host Cell : HEK293
| Cat. No. | CLOE-1370 |
| Introduction | This cell lysate over-expresses C-terminal polyhistidine tagged human CST5 (NP_001891.2) (Met 1-Val 142). Purification of target protein (s) from this cell lysate has been verified. |
| Target Gene | CST5 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | CST5 cystatin D [ Homo sapiens ] |
| Gene Symbol | CST5 |
| Synonyms | CST5; cystatin D; cystatin-D; cystatin 5; cystatin-5; cysteine-proteinase inhibitor; MGC71922; |
| Gene ID | 1473 |
| Uni Prot ID | P28325 |
| m RNA Refseq | BC069514 |
| Chromosome Location | 20p11.21 |
| Function | cysteine-type endopeptidase inhibitor activity; peptidase inhibitor activity; protein binding; |
| Pathway | Endochondral Ossification, organism-specific biosystem; Salivary secretion, organism-specific biosystem; Salivary secretion, conserved biosystem; |
| MIM | 123858 |
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