Transfected Stable Cell Lines
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Cat. No. : CLOE-0771 Host Cell : HEK293
| Cat. No. | CLOE-0771 |
| Description | This cell lysate over-expresses his tagged human COL2A1 (P02458-2) (Asp 1242-Leu 1487),. Purification of target protein (s) from this cell lysate has been verified. |
| Introduction | This cell lysate over-expresses his tagged human COL2A1 (P02458-2) (Asp 1242-Leu 1487),. Purification of target protein (s) from this cell lysate has been verified. |
| Target Gene | COL2A1 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | COL2A1 collagen, type II, alpha 1 [ Homo sapiens ] |
| Gene Symbol | COL2A1 |
| Synonyms | AOM; ANFH; SEDC; COL11A3 |
| Gene Description | collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphyseal dysplasia, congenital) |
| GeneID | 1280 |
| Uni ProtID | P02458 |
| mRNA Refseq | NM_001844.4 |
| Protein Refseq | NP_001835.3 |
| Chromosome Location | 12q13.11 |
| Function | extracellular matrix structural constituent conferring tensile strength; identical protein binding; platelet-derived growth factor binding; |
| Pathway | Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Axon guidance, organism-specific biosystem; Collagen biosynthesis and modifying enzymes, organism-specific biosystem; Collagen formation, organism-specific biosystem; Developmental Biology, organism-specific biosystem; ECM-receptor interaction, organism-specific biosystem; |
| MIM | 120140 |
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