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Human CHEK2 Knockout Cell Line with TP53 wild type background -HCT116

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT0080

Target Gene :   CHEK2 Host Cell :   HCT116

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RT0080
Description HCT116 -CHEK2 (-/-) is a cell line with a homozygous knockout of human CHEK2; TP53 wild type background
Target Gene CHEK2
Host Cell HCT116
Host Cell Species Homo sapiens (Human)
Size >1x106 cells/vial
Validation Sequencing
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name CHEK2 checkpoint kinase 2 [ Homo sapiens ]
Gene Symbol CHEK2
Synonyms CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Gene Description CHK2 checkpoint homolog (S. pombe)
GeneID 11200
Uni ProtID O96017
mRNA Refseq NM_007194.3
Protein Refseq NP_009125.1
Chromosome Location 22q12.1
Function ATP binding; metal ion binding; protein binding; protein homodimerization activity; protein kinase binding; protein serine/threonine kinase activity; protein serine/threonine kinase activity; ubiquitin protein ligase binding;
Pathway Cell Cycle, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, conserved biosystem; DNA damage response, organism-specific biosystem; FOXM1 transcription factor network, organism-specific biosystem;
MIM 604373
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Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
Clear protocol

Highly effective gene knockout reagent with easy-to-follow protocol - would highly recommend.

United States

Work well

I was very impressed with Human CHEK2 Knockout Cell Line with TP53 wild type background -HCT116 it worked extremely well in knocking out my target gene with high efficiency.

United States

Effective

Human CHEK2 Knockout Cell Line with TP53 wild type background -HCT116 exceeded my expectations - it was highly effective and easy to use.

United States

Quickly and effeciently

I was very impressed with how quickly and efficiently Human CHEK2 Knockout Cell Line with TP53 wild type background -HCT116 knocked out my target gene.

United States

Outstanding

As a researcher, I've used many gene knockout reagents, but Human CHEK2 Knockout Cell Line with TP53 wild type background -HCT116 stands out for its reliability and efficiency.

United States

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