Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RT0080
Target Gene : CHEK2 Host Cell : HCT116
Size : >1x106 cells/vial Validation : Sequencing
| Cat. No. | CSC-RT0080 |
| Description | HCT116 -CHEK2 (-/-) is a cell line with a homozygous knockout of human CHEK2; TP53 wild type background |
| Target Gene | CHEK2 |
| Host Cell | HCT116 |
| Host Cell Species | Homo sapiens (Human) |
| Size | >1x106 cells/vial |
| Validation | Sequencing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Gene Name | CHEK2 checkpoint kinase 2 [ Homo sapiens ] |
| Gene Symbol | CHEK2 |
| Synonyms | CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425 |
| Gene Description | CHK2 checkpoint homolog (S. pombe) |
| GeneID | 11200 |
| Uni ProtID | O96017 |
| mRNA Refseq | NM_007194.3 |
| Protein Refseq | NP_009125.1 |
| Chromosome Location | 22q12.1 |
| Function | ATP binding; metal ion binding; protein binding; protein homodimerization activity; protein kinase binding; protein serine/threonine kinase activity; protein serine/threonine kinase activity; ubiquitin protein ligase binding; |
| Pathway | Cell Cycle, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, conserved biosystem; DNA damage response, organism-specific biosystem; FOXM1 transcription factor network, organism-specific biosystem; |
| MIM | 604373 |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
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Highly effective gene knockout reagent with easy-to-follow protocol - would highly recommend.
I was very impressed with Human CHEK2 Knockout Cell Line with TP53 wild type background -HCT116 it worked extremely well in knocking out my target gene with high efficiency.
Human CHEK2 Knockout Cell Line with TP53 wild type background -HCT116 exceeded my expectations - it was highly effective and easy to use.
I was very impressed with how quickly and efficiently Human CHEK2 Knockout Cell Line with TP53 wild type background -HCT116 knocked out my target gene.
As a researcher, I've used many gene knockout reagents, but Human CHEK2 Knockout Cell Line with TP53 wild type background -HCT116 stands out for its reliability and efficiency.
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