Human CFD HEK293 Cell Lysate

Name: Human CFD HEK293 Cell Lysate
SKU: CLOE-1682
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1682 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1682
Introduction	This cell lysate over-expresses the human CFD (P00746) (Ile 26-Ala 253) fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	CFD
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	CFD complement factor D (adipsin) [ Homo sapiens ]
Gene Symbol	CFD
Synonyms	DF; ADN; PFD; ADIPSIN
Gene Description	complement factor D (adipsin)
GeneID	1675
Uni ProtID	P00746
mRNA Refseq	NM_001928.2
Protein Refseq	NP_001919.2
Chromosome Location	19p13.3
Function	serine-type endopeptidase activity; serine-type peptidase activity;
Pathway	Adipogenesis, organism-specific biosystem; Alternative complement activation, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Complement cascade, organism-specific biosystem; Hemostasis, organism-specific biosystem; Immune System, organism-specific biosystem;
MIM	134350

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