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Human CDKN1A Knockout Cell Line-DLD-1

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT0021

Target Gene :   CDKN1A Host Cell :   DLD-1

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RT0021
Description DLD-1-CDKN1A (-/-) is a cell line with a homozygous knockout of human CDKN1A
Target Gene CDKN1A
Host Cell DLD-1
Host Cell Species Homo sapiens (Human)
Size >1x106 cells/vial
Validation Sequencing
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name CDKN1A cyclin-dependent kinase inhibitor 1A (p21, Cip1) [ Homo sapiens ]
Gene Symbol CDKN1A
Synonyms CDKN1A; cyclin-dependent kinase inhibitor 1A (p21, Cip1); CDKN1; cyclin-dependent kinase inhibitor 1; CAP20; CIP1; P21; p21CIP1; p21Cip1/Waf1; SDI1; WAF1; DNA synthesis inhibitor; CDK-interacting protein 1; CDK-interaction protein 1; wild-type p53-activated fragment 1; melanoma differentiation associated protein 6; MDA-6;
GeneID 1026
Uni ProtID P38936
mRNA Refseq BC000275
Chromosome Location 6p21.1
Function cyclin binding; cyclin-dependent protein kinase activating kinase activity; cyclin-dependent protein kinase activity; cyclin-dependent protein kinas
Pathway AKT phosphorylates targets in the cytosol, organism-specific biosystem; AMPK signaling, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Adipogenesis, organism-specific biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; Bladder cancer, organism-specific biosystem;
MIM 116899
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Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
Accurate results

Almost all of the transfection reagents ordered by our group are from this company and I am very confident in the results produced by this reagent

United States

Reliable results

I think the results of this reagent transfection are still relatively reliable and the reproducibility of the results of multiple experiments is good, it is something that can be repurchased next time.

United States

Results deserve recognition

The transfections were efficient and the results of the multiple culture transfections were also very efficient and were presented at the group meeting to the approval of the teachers.

United States

Obvious results

The transfection steps are simple and comparable to those I have used before, and the results are obvious.

United States

Easy to do

The reagents from the previous build I have left unused, it is advisable to choose the right reagents at the beginning, it takes some luck to do experiments, I am now using this reagents with consistently good results.

United States

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