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Cat.No. : CSC-RT0021
Host Cell: DLD-1 Target Gene: CDKN1A
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0021 |
| Description | DLD-1-CDKN1A (-/-) is a cell line with a homozygous knockout of human CDKN1A |
| Target Gene | CDKN1A |
| Host Cell | DLD-1 |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Almost all of the transfection reagents ordered by our group are from this company and I am very confident in the results produced by this reagent
United States
02/25/2023
I think the results of this reagent transfection are still relatively reliable and the reproducibility of the results of multiple experiments is good, it is something that can be repurchased next time.
United States
03/18/2023
The transfections were efficient and the results of the multiple culture transfections were also very efficient and were presented at the group meeting to the approval of the teachers.
United States
03/26/2023
The transfection steps are simple and comparable to those I have used before, and the results are obvious.
United States
02/24/2023
The reagents from the previous build I have left unused, it is advisable to choose the right reagents at the beginning, it takes some luck to do experiments, I am now using this reagents with consistently good results.
United States
03/19/2023
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