Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-1075 Host Cell : HEK293
| Cat. No. | CLOE-1075 |
| Introduction | This cell lysate over-expresses the extracellular domain of human CD8A (NP_001759.3) (Met 1-Asp 182) fused with a polyhistidine tag at the C-terminus. Purification of target protein (s) from this cell lysate has been verified. |
| Target Gene | CD8A |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | CD8A CD8a molecule [ Homo sapiens ] |
| Gene Symbol | CD8A |
| Synonyms | CD8; MAL; p32; Leu2 |
| Gene ID | 925 |
| Uni Prot ID | P01732 |
| m RNA Refseq | NM_001145873.1 |
| Protein Refseq | NP_001139345.1 |
| Chromosome Location | 2p12 |
| Function | MHC class I protein binding; coreceptor activity; protein binding; |
| Pathway | Adaptive Immune System, organism-specific biosystem; Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Downstream signaling in naive CD8+ T cells, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem; |
| MIM | 186910 |
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