Human CD14 HEK293 Cell Lysate

Name: Human CD14 HEK293 Cell Lysate
SKU: CLOE-1755
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1755 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1755
Introduction	This cell lysate over-expresses the human CD14 (NP_000582.1) (Thr 20-Cys 352) fused with the Fc region of human IgG1 at the N-terminus. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	CD14
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	CD14 CD14 molecule [ Homo sapiens ]
Gene Symbol	CD14
Synonyms	CD14; CD14 molecule; CD14 antigen; monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein;
GeneID	929
Uni ProtID	P08571
mRNA Refseq	BC010507
Chromosome Location	5q22-q32
Function	lipopolysaccharide binding; lipoteichoic acid binding; opsonin receptor activity; peptidoglycan receptor activity;
Pathway	Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; LPS transferred from LBP carrier to CD14, organism-specific biosystem;
MIM	158120

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