Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-1730 Host Cell : HEK293
| Cat. No. | CLOE-1730 |
| Introduction | This cell lysate over-expresses C-terminal polyhistidine tagged human CA9 (NP_001207.2) (Met 1-Asp 414). Purification of target protein (s) from this cell lysate has been verified. |
| Target Gene | CA9 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | CA9 carbonic anhydrase IX [ Homo sapiens ] |
| Gene Symbol | CA9 |
| Synonyms | CA9; carbonic anhydrase IX; carbonic anhydrase 9; CAIX; carbonic dehydratase; MN; RCC associated protein G250; pMW1; CA-IX; P54/58N; membrane antigen MN; carbonate dehydratase IX; RCC-associated antigen G250; RCC-associated protein G250; renal cell carcinoma-associated antigen G250; |
| GeneID | 768 |
| Uni ProtID | Q16790 |
| mRNA Refseq | BC014950 |
| Chromosome Location | 9p12 |
| Function | carbonate dehydratase activity; lyase activity; metal ion binding; zinc ion binding; |
| Pathway | HIF-1-alpha transcription factor network, organism-specific biosystem; |
| MIM | 603179 |
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