Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-1392 Host Cell : HEK293
| Cat. No. | CLOE-1392 |
| Introduction | This cell lysate over-expresses C-terminal polyhistidine tagged human CA4 (NP_000708.1) (Met1-Lys283). Purification of target protein (s) from this cell lysate has been verified. |
| Target Gene | CA4 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | CA4 carbonic anhydrase IV [ Homo sapiens ] |
| Gene Symbol | CA4 |
| Synonyms | CA4; carbonic anhydrase IV; retinitis pigmentosa 17 (autosomal dominant) , RP17; carbonic anhydrase 4; CAIV; Car4; CA-IV; carbonic dehydratase IV; carbonate dehydratase IV; RP17; |
| GeneID | 762 |
| Uni ProtID | P22748 |
| mRNA Refseq | BC057792 |
| Chromosome Location | 17q23 |
| Function | carbonate dehydratase activity; lyase activity; metal ion binding; protein binding; zinc ion binding; |
| Pathway | Proximal tubule bicarbonate reclamation, organism-specific biosystem; Proximal tubule bicarbonate reclamation, conserved biosystem; |
| MIM | 114760 |
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