Human BTRC Knockout Cell Line-HEK293T

Name: Human BTRC Knockout Cell Line-HEK293T
SKU: CSC-RT2345
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2345

Target Gene : BTRC Host Cell : HEK293T

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2345
Description	This cell is a stable cell line with a homozygous knockout of human BTRC using CRISPR/Cas9.
Target Gene	BTRC
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Size	>1x10⁶ cells/vial
Validation	Sequencing
Storage	Liquid nirtogen

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	BTRC beta-transducin repeat containing E3 ubiquitin protein ligase [ Homo sapiens ]
Gene Symbol	BTRC
Synonyms	BTRC; beta-transducin repeat containing E3 ubiquitin protein ligase; beta transducin repeat containing; F-box/WD repeat-containing protein 1A; beta TrCP1; betaTrCP; bTrCP; bTrCP1; FBXW1A; Fwd1; beta-TrCP1; E3RSIkappaB; F-box and WD-repeat protein 1B; pIkappaBalpha-E3 receptor subunit; F-box and WD repeats protein beta-TrCP; FWD1; FBW1A; FBXW1; BETA-TRCP; MGC4643;
Gene ID	8945
Uni Prot ID	Q9Y297
m RNA Refseq	BC027994
Chromosome Location	10q24.32
Function	beta-catenin binding; ligase activity; protein binding; protein dimerization activi
Pathway	APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; Activation of NF-kappaB in B Cells, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Alternative NF-kappaB pathway, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Atypical NF-kappaB pathway, organism-specific biosystem;
MIM	603482

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What is the recommended growth medium? Does it require antibiotic selection?

A: DMEM supplemented with 10% fetal bovine serum. <br> It is not required to add the selection antibiotics when culturing the KO cells.

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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