Human BTD HEK293 Cell Lysate

Name: Human BTD HEK293 Cell Lysate
SKU: CLOE-0131
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-0131 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-0131
Description	This cell lysate over-expresses his tagged human BTD (P43251) (Met1-Asp543). Purification of target protein (s) from this cell lysate has been verified.
Introduction	This cell lysate over-expresses his tagged human BTD (P43251) (Met1-Asp543). Purification of target protein (s) from this cell lysate has been verified.
Target Gene	BTD
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	BTD biotinidase [ Homo sapiens ]
Gene Symbol	BTD
Gene Description	biotinidase
GeneID	686
Uni ProtID	P43251
mRNA Refseq	NM_000060.2
Protein Refseq	NP_000051.1
Chromosome Location	3p25
Function	biotin carboxylase activity; biotinidase activity;
Pathway	Biotin metabolism, organism-specific biosystem; Biotin metabolism, conserved biosystem; Vitamin digestion and absorption, organism-specific biosystem; Vitamin digestion and absorption, conserved biosystem;
MIM	609019

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