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Cat.No. : CSC-RT0004
Host Cell: DLD-1 Target Gene: BAX/BAK
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0004 |
| Description | DLD-1-BAX/BAK (-/-,-/-) is a cell line with a double homozygous knockout of human BAX and BAK |
| Target Gene | BAX/BAK |
| Host Cell | DLD-1 |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The difference in the amount of reagent used is immediately shown in the test results and is more accurate.
United States
02/25/2023
This is a very important gene in my experimental design, such an efficient and cost-effective product is really very resource-efficient.
United States
12/02/2021
This gene is a star molecule that must be detected in the gene for detecting apoptosis, and the effect is gratifying with this product.
United States
02/06/2022
I have done many knockout experiments on this gene and used many products, and the effect of this product is the most unexpected and the sense of achievement is very strong.
United States
09/10/2022
I think the experiments on gene knockouts were brilliantly designed throughout the experimental framework, and I'm so grateful that I found the perfect knockout reagent
United States
01/18/2023
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