Human B4GALT1 HEK293 Cell Lysate

Name: Human B4GALT1 HEK293 Cell Lysate
SKU: CLOE-0947
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-0947 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-0947
Introduction	This cell lysate over-expresses extracellular domain of human B4GALT1 (NP_001488.2) (Gly 44-Ser 398) fused with a polyhistidine tag at the N-terminus. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	B4GALT1
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	B4GALT1 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 [ Homo sapiens ]
Gene Symbol	B4GALT1
Synonyms	GT1; GTB; CDG2D; GGTB2; B4GAL-T1; beta4Gal-T1
Gene ID	2683
Uni Prot ID	P15291
m RNA Refseq	NM_001497.3
Protein Refseq	NP_001488.2
Chromosome Location	9p13
Function	N-acetyllactosamine synthase activity; UDP-galactosyltransferase activity; alpha-tubulin binding; beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity; beta-tubulin binding; cytoskeletal protein binding; galactosyltransferase activity; lactose synthase activity; metal ion binding; protein homodimerization activity;
Pathway	Asparagine N-linked glycosylation, organism-specific biosystem; Disease, organism-specific biosystem; Galactose metabolism, organism-specific biosystem; Galactose metabolism, conserved biosystem; Glycosaminoglycan biosynthesis - keratan sulfate, organism-specific biosystem; Glycosaminoglycan biosynthesis - keratan sulfate, conserved biosystem; Glycosaminoglycan metabolism, organism-specific biosystem;
MIM	137060

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