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Human ARF1 Knockout Cell Line-MCF7

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT2087

Target Gene :   ARF1 Host Cell :   MCF7

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RT2087
Description This cell is a stable cell line with a homozygous knockout of human ARF1 using CRISPR/Cas9.
Target Gene ARF1
Host Cell MCF7
Host Cell Species Homo sapiens (Human)
Size >1x106 cells/vial
Validation Sequencing
Storage Liquid nirtogen
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name ARF1 ADP-ribosylation factor 1 [ Homo sapiens ]
Gene Symbol ARF1
Gene Description ADP-ribosylation factor 1
GeneID 375
Uni ProtID P84077
mRNA Refseq NM_001024226.1
Protein Refseq NP_001019397.1
Chromosome Location 1q42
Function GTP binding; GTPase activity; protein binding; receptor signaling protein activity;
Pathway Adaptive Immune System, organism-specific biosystem; Arf1 pathway, organism-specific biosystem; Arf6 downstream pathway, organism-specific biosystem; COPI Mediated Transport, organism-specific biosystem; Class I PI3K signaling events, organism-specific biosystem; Clathrin derived vesicle budding, organism-specific biosystem; Disease, organism-specific biosystem;
MIM 103180
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Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
Reliable and efficient

Soon after I placed the order, I received the product and used it in the experiment. The cell activity was high, which was good!

United States

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