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Human APOC1 Knockout Cell Line-HCT116

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT0048

Target Gene :   APOC1 Host Cell :   HCT116

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RT0048
Description HCT116-APOC1 (-/-) is a cell line with a homozygous knockout of human APOC1
Target Gene APOC1
Host Cell HCT116
Host Cell Species Homo sapiens (Human)
Size >1x106 cells/vial
Validation Sequencing
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name APOC1 apolipoprotein C-I [ Homo sapiens ]
Gene Symbol APOC1
Gene Description apolipoprotein C-I
Gene ID 341
Uni Prot ID B2R526
m RNA Refseq NM_001645.3
Protein Refseq NP_001636.1
Chromosome Location 19q13.2
Function fatty acid binding; lipase inhibitor activity; phosphatidylcholine binding; phosphatidylcholine-sterol O-acyltransferase activator activity; phospholipase inhibitor activity;
Pathway Statin Pathway, organism-specific biosystem;
MIM 107710
Quick Inquiry

Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
Fast & Approximate

This is a good reagent for transfection, I can see the results clearly by shooting fluorescence, and I can finish the test efficiently

United States

Efficient

Good and easy to use, not so many tedious steps, saves time and effort, cheap and saves money for the subject group.

United States

Available results

I recommend this reagent to everyone because it gives really surprising results. And it's also cheap, so you definitely won't lose out.

United States

Easy to do

It is efficient and saves a lot of time. The reagents in the previous build kept knocking out incompletely, making it impossible to continue with later experiments, and this one knocked out completely.

United States

Cheap but useful

I must say that this reagent is really cheap, but it turns out so surprisingly well. Recommended for everyone.

United States

High quality

The APOCL knockout cell line of a brand is of extremely high quality, easy to use, and can accurately carry out gene knockout experiments.

United States

Reliable results

Their cell lines are very stable and can ensure the reliability and repeatability of the experimental results.

United States

Satisfied

The brand's APOCL knockout cell line is easy to use and effective. I have used cell lines of other brands before, but the effect is not satisfactory. But when I tried the cell lines of this brand, I was very surprised at their efficiency and accuracy. Since then, I have been using this brand of APOC1 knockout cell line, and I have always been very satisfied.

United States

High quality

The brand's APOC1 knockout cell line are very reliable in quality and can provide stable and repeatable experimental results.

United States

Reassured by the results

I am very relieved every time I use their cell lines, because I know that I can get accurate and reliable data. This allows me to better advance the experiment in my research.

United States

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