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Stable expression over 15 generations with rapid cell line development in just 3 months.
Supports adherent and suspension cell lines, offering MCB, WCB, and PCB establishment.
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Our plasmid production services span Non-GMP, GMP-Like, and GMP-Grade levels, with specialized options for linearized plasmids.
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Advanced platforms for AAV, adenovirus, lentivirus, and retrovirus production, with strict adherence to GMP guidelines and robust quality control.
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Leverage AI to uncover hidden high-potential small molecules, prioritize leads intelligently, and reduce costly trial-and-error in early drug discovery.
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Use AI-guided design to optimize protein degraders, addressing design complexity and enhancing efficacy while shortening development timelines.
Cat.No. : HPAK-01
| Cat. No. | HPAK-01 |
| Description | Recombinant adeno-associated virus (AAV) vectors are promising tools for in vivo and in vitro gene transfer. Creative Biogene's High-purity™ AAV purification kits provide a fast and easy procedure for purifying AAV particles from cell lysates. This column-based kit is suitable for different serotypes and has shown good performance in both yield and purity. |
| Applications | AAV Purification |
| Serotype(s) | Most serotypes (such as AAV2, AAV5, AAV6, AAV8, AAV9, etc.) |
| Input Type | Cell fraction, media fraction, or mixed cells and media |
| Loading Capacity | ≥10^14 VP |
| Recovery Rate | ＞80% |
| Features |
(1) Fast and easy to operate (2) No expensive equipment required (3) High AAV recovery rate, up to 90% (4) Can be used for multiple AAV serotypes (5) Obtain highly active AAV for in vitro experiments (6) After being reused more than 50 times, the recovery rate is still good (7) Multiple purifications can be done in parallel for rapid screening experiments |
| Storage | Store all components at 2-8°C |
Figure 1. AAV8 virus particle purification.
A: AAV stands for adeno-associated virus, which is a small, non-enveloped virus that infects humans and some other animals. AAV is commonly used as a vector for gene therapy and gene delivery experiments in research and medical settings.
A: AAV purification is the process of isolating and purifying adeno-associated viruses (AAVs) from a mixture of other cellular components and contaminants.
A: The purification process typically involves several steps, including cell lysis, filtration, chromatography, and ultracentrifugation. The specific techniques used may vary depending on the desired level of purity and the specific characteristics of the AAVs being purified.
A: The goal of AAV purification is to obtain a highly pure and concentrated viral solution that can be used for research, pre-clinical, or clinical applications.
A: Purified AAVs are essential for ensuring the safety and efficacy of gene therapy treatments and other applications that rely on the delivery of genetic material to cells.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
By using these kits, we can simplify the purification process, improve virus recovery, and obtain highly pure AAV particles for downstream applications.
United States
11/03/2022
AAV purification kits offer a degree of flexibility, enabling researchers to adapt the purification process to their specific needs. I like them very much.
United Kingdom
02/23/2023
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