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HNRNPA1 Knockout Cell Line-HCT116

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT0102

Target Gene :   HNRNPA1 Host Cell :   HCT116

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No. CSC-RT0102
Description HCT116-HNRNPA1 cell line is a stable cell line with a homozygous knockout of HNRNPA1
Target Gene HNRNPA1
Host Cell HCT116
Host Cell Species Homo sapiens (Human)
Size >1x106 cells/vial
Validation Sequencing
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Quick Inquiry

Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
Exceeded my expectations

HNRNPA1 Knockout Cell Line-HCT116 has truly exceeded my expectations. Its exceptional knockout efficiency and reliable performance have propelled my research to new heights, enabling groundbreaking discoveries.

France

Highly impressed

I am highly impressed with the reliability of HNRNPA1 Knockout Cell Line-HCT116. Its consistent knockout outcomes have provided me with confidence in the accuracy and reproducibility of my experimental results.

United States

Achieve targeted gene modifications with ease

Using HNRNPA1 Knockout Cell Line-HCT116 has been a outstanding helper for my research endeavors. Its ease of use and exceptional knockout efficiency have allowed me to achieve targeted gene modifications with ease.

United States

Exceptional performance

I cannot emphasize enough how valuable HNRNPA1 Knockout Cell Line-HCT116 has been for unraveling the functional significance of specific genes. Its exceptional performance has enabled me to uncover critical insights into biological processes.

United States

Indispensable tool

HNRNPA1 Knockout Cell Line-HCT116 has become an indispensable tool in my research toolkit. Its outstanding knockout efficiency, combined with its user-friendly design, has significantly streamlined my experimental workflow.

United States

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