Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RT0270
Target Gene : HMGN2 Host Cell : 293T
Size : >1x106 cells/vial Validation : Sequencing
| Cat. No. | CSC-RT0270 |
| Description | 293T-HMGN2 (-/-) is a stable cell line with a homozygous knockout of human HMGN2 |
| Background | The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMGN1, the encoded protein may help maintain an open chromatin configuration around transcribable genes. The protein has also been found to have antimicrobial activity against bacteria, viruses and fungi. [provided by RefSeq, Oct 2014] |
| Target Gene | HMGN2 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Size | >1x106 cells/vial |
| Validation | Sequencing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
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HMGN2 Knockout Cell Line-293T's impact on my research is profound, revealing the intricacies of gene functions.
HMGN2 Knockout Cell Line-293T's efficiency ensures I can explore diverse genetic questions with accuracy and speed.
HMGN2 Knockout Cell Line-293T elevates my work, contributing to a comprehensive understanding of genetics.
HMGN2 Knockout Cell Line-293T's user-friendly interface simplifies gene editing, making it accessible to researchers of all levels.
Through consistent performance, HMGN2 Knockout Cell Line-293T fuels my curiosity and passion for genetic exploration.
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