HADHA Knockout Cell Line-293T

Name: HADHA Knockout Cell Line-293T
SKU: CSC-RT0246
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT0246

Target Gene : HADHA Host Cell : 293T

Size : >1x10⁶ cells/vial Validation : Sequencing

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RT0246
Description	293T-HADHA (-/-) is a stable cell line with a homozygous knockout of human HADHA
Background	This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. [provided by RefSeq, Jul 2008]
Target Gene	HADHA
Host Cell	293T
Host Cell Species	Homo sapiens (Human)
Size	>1x10⁶ cells/vial
Validation	Sequencing

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Customer Reviews

Advanced

Using HADHA Knockout Cell Line-293T has significantly advanced my genetic research, providing a reliable and effective solution for precise gene editing and unraveling the complexities of gene function.

United States

2021-10-09

Remarkable

I am impressed by the remarkable efficiency of HADHA Knockout Cell Line-293T, enabling me to achieve targeted gene modifications with exceptional accuracy and reproducibility.

United States

2022-01-27

Clear

The easy to finish protocol and clear instructions accompanying HADHA Knockout Cell Line-293T have made it accessible to researchers of all levels, facilitating seamless integration into my experimental workflow.

United States

2022-07-19

Robust

The consistent and robust results obtained using HADHA Knockout Cell Line-293T have provided solid evidence to support my research hypotheses and advance our understanding of gene function.

United States

2022-11-03

Appreciate

I appreciate the excellent customer support provided by the manufacturer, who has been responsive, knowledgeable, and instrumental in ensuring the success of my gene knockout experiments.

United Kingdom

2023-02-23

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *

Name

Country *

Email *

Related Products

Cat.No.	Product Name	Price
CSC-RK0053	Nos3 Knockout Mouse Lung Fibroblasts	Inquiry
CSC-RK0076	Tlr4 Knockout Mouse Embryonic Fibroblasts	Inquiry
CSC-RK0080	Tlr4 Knockout Mouse Spleen Cells	Inquiry
CSC-RK0099	Icam1 Knockout Mouse Liver Cells	Inquiry
CSC-RK0100	Icam1 Knockout Mouse Spleen Cells	Inquiry
CSC-RK0064	Nos3 Knockout Mouse Lung Cells	Inquiry
CSC-RK0111	Cybb Knockout Mouse Bone Marrow Mesenchymal Stem Cells	Inquiry
CSC-RK0062	Nos3 Knockout Mouse Liver Cells	Inquiry
CSC-RK0110	Cybb Knockout Mouse Bone Marrow Neutrophils	Inquiry
CSC-RK0114	Cybb Knockout Mouse Heart Cells	Inquiry

HADHA Knockout Cell Line-293T

Cell Line Information

Cell Culture Information

Safety and Packaging

Q & A

Customer Reviews

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

Advanced

Remarkable

Clear

Robust

Appreciate

Related Products

Related Services