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Cat.No. : CSC-RT0263
Host Cell: 293T Target Gene: GSTO1
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0263 |
| Description | 293T-GSTO1 (-/-) is a stable cell line with a homozygous knockout of human GSTO1 |
| Background | The protein encoded by this gene is an omega class glutathione S-transferase (GST) with glutathione-dependent thiol transferase and dehydroascorbate reductase activities. GSTs are involved in the metabolism of xenobiotics and carcinogens. The encoded protein acts as a homodimer and is found in the cytoplasm. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010] |
| Target Gene | GSTO1 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The practical advantages of GSTO1 Knockout Cell Line-293T extend beyond the lab, with its potential implications in therapeutic development, agriculture, and biotechnology industries offering promise for real-world applications.
United States
05/12/2021
GSTO1 Knockout Cell Line-293T's effectiveness in generating gene knockouts has been a game-changer in understanding the genetic basis of diseases, offering hope for targeted therapies and personalized medicine approaches.
United States
08/15/2021
GSTO1 Knockout Cell Line-293T's ease of use and efficiency have also been critical in educational settings, empowering students and aspiring researchers to gain hands-on experience in gene editing techniques and fostering future talent in genetics research.
United States
10/09/2021
GSTO1 Knockout Cell Line-293T's role in elucidating gene functions has led to the identification of potential drug targets, expediting drug development and fostering progress in the pharmaceutical industry.
United States
01/27/2022
The reliability of GSTO1 Knockout Cell Line-293T has proven invaluable in biotechnology, facilitating the production of genetically modified organisms with enhanced traits and commercial applications.
Germany
07/19/2022
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