Cat.No. : CSC-RR0124 Host Cell: SW480
| Cat. No. | CSC-RR0124 |
| Description | SW480 was derived from colon of a 50 year-old male with colorectal adenocarcinoma. SW480 is negative for CSAp (CSAp-) and colon antigen 3 and are positive for keratin by immunoperoxidase staining. There is a G to A mutation in codon 273 of the p53 gene resulting in an Arg to His substitution and a C to T mutation in codon 309 resulting in a Pro to Ser substitution. SW480 cells express elevated levels of p53 protein. The line is positive for expression of c-myc, K-ras, H-ras, N-ras, myb, sis and fos oncogenes. N-myc oncogene expression was not detected. Matrilysin, a metalloproteinase associated with tumor invasiveness, is not expressed. SW480 cells have been reported to produce GM-CSF. The GFP Stable Cell Line-SW480 constitutively expresses GFP. |
| Host Cell | SW480 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Recommended Medium | Inquiry for instruction of culturing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
The immune system and tumor cells interact dynamically in a process known as cancer immunoediting. Using a zebrafish xenograft model, the researchers analyze the dynamics of cancer immunoediting using the Luc Reporter Cell Line-B16F10 cell line. This cell line enables luciferase-based bioluminescence imaging to be used for real-time monitoring of tumor progression and immune cell interactions. By examining the interactions between distinct subtypes of colorectal cancer cells and innate immune cells, such as neutrophils and macrophages, they clarify the mechanisms involved in tumor clearance as opposed to progression. The study's results emphasize the function of myeloid cells in this procedure and propose zebrafish xenografts as possible indicators for evaluating the dynamics of the tumor microenvironment.
Figure 1. By using GFP-transfected parental cells injected into tumors at 1 and 4 dpi for scRNAseq analysis, the researchers used Luc Reporter Cell Line-B16F10 to investigate molecular alterations underlying SW480 escapers by single-cell transcriptome profiling. (Póvoa V, et al, 2021)
A: Our GFP Stable Cell Line-SW480 has undergone strict screening and verification to ensure the stable expression of the GFP gene. Through methods such as fluorescence microscopy and flow cytometry, we verified the expression level of GFP and performed repeated tests at different time points and in different batches. Cells can maintain stable GFP expression under appropriate culture conditions.
A: The impact of the SW480 cell line as a vector on GFP stable cell lines is considered to be minimal. However, we recommend that customers pay attention to the special growth requirements of the SW480 cell line during use, such as culture medium components and culture conditions, to ensure optimal expression and stability of the cells. We provide detailed training guides to help clients overcome potential issues.
A: Yes, our GFP Stable Cell Line-SW480 has passed strict quality control inspection. We verify cell lines according to international and internal standards, including stability of gene expression, cell morphology, contaminant detection, etc. We ensure that each batch of cell lines meets our quality standards to ensure our customers receive a high-quality product.
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The GFP stable cell line-SW480 does have good fluorescent labeling function as described. During the experiment, the growth of the cells was generally stable and the gene expression was relatively consistent, which still met our research needs.
The GFP stable cell line-SW480 of this platform performs well overall. The cells grew well under standard culture conditions and the cell morphology was relatively uniform. The expression of fluorescent proteins is relatively stable and suitable for research on tracking cell movement and gene expression.
I have been using the GFP stable cell line-SW480 provided by this supplier for some time, and overall the experience is moderate. The fluorescence intensity is stable and suitable for short-term experiments. Pay attention to changes in fluorescence intensity during long-term culture. I'll observe again.
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