Cat.No. : CSC-RR0115 Host Cell: Raji
| Cat. No. | CSC-RR0115 |
| Description | The Raji line of lymphoblast-like cells was established by R.J.V. Pulvertaft in 1963 from a Burkitt's lymphoma of the left maxilla of an 11-year-old Black male. These cells are EBNA positive and are partially resistant to poliovirus and vesicular stomatitis viruses. The GFP Stable Cell Line-Raji constitutively expresses GFP. |
| Host Cell | Raji |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Recommended Medium | Inquiry for instruction of culturing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Many patients with refractory or relapsed B-cell malignancies have achieved complete remission after receiving T cells redirected with chimeric antigen receptors (CARs) targeting the pan-B-cell antigen CD19. The researchers used T cells containing CD7-specific CARs and discovered that these cells underwent fratricide due to residual CD7 expression, which impeded their expansion. To prevent this fratricide, the researchers performed targeted genomic disruption of the CD7 gene, successfully avoiding fratricide and enabling the expansion of CD7 CAR T cells without compromising their cytotoxic function. They demonstrated that CD7 CAR T cells exhibited strong cytotoxicity against malignant T-cell lines and primary tumors and provided protection in a T-ALL mouse xenograft model. Although CD7 CAR T cells were also toxic to unedited (CD7+) T cells and NK cells, the researchers showed that CD7-edited T cells could still respond to viral peptides and potentially offer protection against pathogens. By disrupting the target antigen at the genomic level, they overcame the fratricide issue in CAR T cells, establishing the feasibility of using CD7 CAR T cells for targeted therapy of T-cell malignancies.
Figure 1. The researchers used electroporation to introduce Cas9 into T cells in combination with CD7-specific or control (CD19-specific) gRNA and transduce CD19 CAR. The frequency of different subtypes of cells in CD19 CAR T cells was evaluated by flow cytometry on day 7 after transduction, as well as the production of TNFα and IFNγ in CD19 CAR T cells in the presence of CD19+ NALM6 cells. GFP+ Raji cells were co-cultured with CD7KO or control CD19 CAR T cells, and the total number of surviving tumor cells and CD19 CAR T cells was measured after 72 hours of co-culture. (Gomes-Silva D, et al., 2017)
A: Raji ell Line is a human B cell line derived from Burkitt lymphoma cells taken from a patient with African malaria. It is widely used in research for studying B cell malignancies and immune responses.
A: While Raji Cell Line is a valuable research tool, it is important to be aware of its tumorigenic potential and handle it with appropriate biosecurity measures. Additionally, its unique characteristics may not fully represent all B cell malignancies, so results need to be interpreted with caution.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
I am very satisfied with the GFP Stable Cell Line-Raji. It is of superior quality and a reliable tool for my experiments. The customer service is also top notch!
The GFP Stable Cell Line-Raji is very robust and easy to handle. It has saved me time and effort in my research. The price is also very reasonable compared to other products.
The GFP Stable Cell Line-Raji has excellent stability and produces consistent results. The customer support team is helpful and responsive and provides excellent technical support.
I highly recommend the GFP Stable Cell Line-Raji for its excellent performance and quality. It has exceeded my expectations and has been an important part of my research.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
