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FreeClone™ Seamless Cloning kit

Creative Biogene’s FreeClone™ Seamless Cloning kit is designed to help researchers to streamline research and workflow, and we have constructed hundreds of clones in high efficiency with FreeClone™ Seamless Cloning kit. FreeClone™ Seamless Cloning kit enables the simultaneous and directional cloning of 2 or more PCR fragments, consisting of any sequence, into any linearized vector, typically in 20 minutes, without extra DNA sequences, restriction endonucleases, or ligation.The only thing requires for the kit is the DNA fragments contain ~15-50 base pair overlap with adjacent DNA fragments.

FreeClone™ Seamless Cloning has many advantages compared to conventional restriction enzyme/ligation cloning.

  1. No restriction digest of the DNA fragments after PCR is necessary. No restriction site scar remains between two DNA fragments.
  2. The backbone vector can be digested, or synthesized by PCR.
  3. It is far simpler than conventional cloning schemes, as it requires fewer steps. It takes only 20 minutes for assembly reaction and transformation.
  4. Multiple DNA fragments can be combined simultaneously in a single-tube reaction.

 

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