Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : SCK-01
| Cat. No. | SCK-01 |
| Description | Provides core reagents for efficient Seamless Cloning |
A: The group of cloning methods we refer to as "seamless cloning" typically combine attributes of more established cloning methods to create a unique solution to allow sequence-independent and scarless insertion of one or more fragments of DNA into a plasmid vector.
A: The ability to quickly join a single insert to a plasmid at any sequence in the vector without a scar, makes these technologies very appealing cloning methods. Additionally, the ability to join 5-10 fragments in a predetermined order, with no sequence restrictions or scars, provides a powerful technique for synthetic biology endeavors, such as moving whole operons for metabolic engineering or whole genome reconstructions.
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The system allows the cloning of the DNA fragments into virtually any linearized Escherichia. coli vector, requires no pre-existing recombination sites or extra DNA sequences, and eliminates the need for extensive enzymatic treatments of the DNA such as restriction and ligation.
This kit is accurate and efficient, and can obtain up to 90% correct clones without leaving additional sequences.
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