Creative Biogene’s FreeClone™ Seamless Cloning kit is designed to help researchers to streamline research and workflow, and we have constructed hundreds of clones in high efficiency with FreeClone™ Seamless Cloning kit. FreeClone™ Seamless Cloning kit enables the simultaneous and directional cloning of 2 or more PCR fragments, consisting of any sequence, into any linearized vector, typically in 20 minutes, without extra DNA sequences, restriction endonucleases, or ligation.The only thing requires for the kit is the DNA fragments contain ~15-50 base pair overlap with adjacent DNA fragments.
FreeClone™ Seamless Cloning has many advantages compared to conventional restriction enzyme/ligation cloning.
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