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FreeClone™ Seamless Cloning kit

For research use only. Not intended for any clinical use.
Cat.No.
SCK-01
Description
Provides core reagents for efficient Seamless Cloning

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Customer Q&As
What is seamless cloning?

A: The group of cloning methods we refer to as "seamless cloning" typically combine attributes of more established cloning methods to create a unique solution to allow sequence-independent and scarless insertion of one or more fragments of DNA into a plasmid vector.

What are the advantages of seamless cloning?

A: The ability to quickly join a single insert to a plasmid at any sequence in the vector without a scar, makes these technologies very appealing cloning methods. Additionally, the ability to join 5-10 fragments in a predetermined order, with no sequence restrictions or scars, provides a powerful technique for synthetic biology endeavors, such as moving whole operons for metabolic engineering or whole genome reconstructions.

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Customer Reviews
Easy to Use

The system allows the cloning of the DNA fragments into virtually any linearized Escherichia. coli vector, requires no pre-existing recombination sites or extra DNA sequences, and eliminates the need for extensive enzymatic treatments of the DNA such as restriction and ligation.

United States

12/03/2022

This Kit Is Accurate And Efficient

This kit is accurate and efficient, and can obtain up to 90% correct clones without leaving additional sequences.

United States

12/03/2022

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