Cat.No. : CSC-RT0345
Host Cell: 293T Target Gene: FXYD5
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0345 |
| Description | 293T-FXYD5 (-/-) is a stable cell line with a homozygous knockout of human FXYD5 |
| Target Gene | FXYD5 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
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The efficiency with which FXYD5 Knockout Cell Line-293T achieves gene knockout has saved me valuable time, allowing me to focus on analyzing results and drawing meaningful conclusions from my experiments.
Through its exceptional performance, FXYD5 Knockout Cell Line-293T has opened up new possibilities for my research, allowing me to explore complex genetic interactions and contribute to a deeper understanding of cellular mechanisms.
The high success rate of gene knockout achieved with FXYD5 Knockout Cell Line-293T has been critical in obtaining statistically robust data, strengthening the scientific rigor of my studies and elevating the impact of my research.
FXYD5 Knockout Cell Line-293T's consistent and reliable performance has also earned praise from my peers, who have recognized the value of this gene knockout reagent and its instrumental role in advancing our collective knowledge in the field of genetics.
In a fast-paced and competitive scientific landscape, FXYD5 Knockout Cell Line-293T has proven to be an invaluable asset, equipping me with the tools necessary to push the boundaries of genetic research and make significant contributions to the scientific community.
FXYD5 Knockout Cell Line-293T's straightforward operation simplifies complex gene editing, optimizing my research efficiency and ensuring accuracy in experiments.
Consistent successful knockouts validate FXYD5 Knockout Cell Line-293T's role as a dependable genetic research tool, reinforcing the credibility and robustness of my findings.
Versatility in applications across multiple research fields promises innovation, progress, and potential breakthroughs in diverse scientific domains.
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