Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RT0233
Target Gene : DRG1 Host Cell : 293T
Size : >1x106 cells/vial Validation : Sequencing
| Cat. No. | CSC-RT0233 |
| Description | 293T-DRG1 (-/-) is a stable cell line with a homozygous knockout of human DRG1 |
| Target Gene | DRG1 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Size | >1x106 cells/vial |
| Validation | Sequencing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
With each experiment, I am reminded of the collaborative efforts that have culminated in the development of DRG1 Knockout Cell Line-293T, an embodiment of the collective pursuit of scientific advancement.
The knowledge gained from using DRG1 Knockout Cell Line-293T has not only enriched my academic journey but has also provided me with a deeper appreciation for the intricate mechanisms that govern life itself.
The profound impact of DRG1 Knockout Cell Line-293T extends far beyond the confines of my laboratory, inspiring me to share my research findings and contribute to the dissemination of scientific knowledge.
As I engage in discussions with fellow researchers, DRG1 Knockout Cell Line-293T's reliability and effectiveness continue to garner admiration and respect, further reinforcing its significance in the scientific community.
In this ever-evolving journey of discovery, I remain grateful for the profound influence of DRG1 Knockout Cell Line-293T, which has not only propelled my research but also ignited a passion for lifelong learning in the captivating world of genetics.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.