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Cat.No. : CSC-RT0088
Host Cell: KYSE30 Target Gene: DDAH2
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0088 |
| Description | KYSE30-DDAH2 cell line is a stable cell line with a homozygous knockout of DDAH2 |
| Target Gene | DDAH2 |
| Host Cell | KYSE30 |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
I am really impressed with DDAH2 Knockout Cell Line-KYSE30. It is highly efficient and consistently produces high-quality results. It has helped me save time and achieve my research goals faster.
United States
08/23/2020
DDAH2 Knockout Cell Line-KYSE30 is an excellent tool for gene editing. It is easy to use and produces knockout efficiencies that are comparable to more expensive reagents.
Germany
05/12/2021
I highly recommend DDAH2 Knockout Cell Line-KYSE30 to any researcher looking for a reliable and efficient tool for gene editing. It has helped me streamline my workflow and achieve excellent results.
United States
08/15/2021
DDAH2 Knockout Cell Line-KYSE30 has become a staple in my lab. It is easy to use, and the knockout efficiency is consistently high. I couldn't be happier with the results.
United States
10/09/2021
I have tried several gene knockout reagents in the past, but this is by far the best. It is user-friendly, efficient, and consistently produces high-quality results. It is a must-have for any gene editing experiment.
France
01/27/2022
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