Cybb Knockout Mouse Lung Cells

Name: Cybb Knockout Mouse Lung Cells
SKU: CSC-RK0118
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RK0118

Target Gene : Cybb Host Cell : Lung cells

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RK0118
Background	The prototype phagaocytic NADPH oxidase is composed of membrane-bound gp91phox and p22hox, as well as cytosolic subunits such as p47(phox), p67(phox) and small GTPase Rac.
Target Gene	Cybb
Abbr	MPLUCs-Cybb Knockout
Alias	CYBB, gp91-1, gp91-phox, CGD91-phox, Cgd, Cyd
Host Cell	Lung cells
Host Cell Species	Mus musculus (Mouse)
Size	>1x10⁶ cells/vial
Validation	Sequencing
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Cybb cytochrome b-245, beta polypeptide [ Mus musculus ]
Gene Symbol	Cybb
Synonyms	Cgd; Cyd; Nox2; C88302; gp91phox
Gene Description	cytochrome b-245, beta polypeptide
Gene ID	13058
Uni Prot ID	Q3U6G0
m RNA Refseq	NM_007807.4
Protein Refseq	NP_031833.3
Chromosome Location	X A1.1; X
Function	flavin adenine dinucleotide binding; heme binding; ion channel activity; metal ion binding; oxidoreductase activity; protein heterodimerization activity; voltage-gated ion channel activity;
Pathway	Adaptive Immune System, organism-specific biosystem; Antigen processing-Cross presentation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Cross-presentation of particulate exogenous antigens (phagosomes), organism-specific biosystem; Disease, organism-specific biosystem; HIF-1 signaling pathway, organism-specific biosystem;

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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