Cat.No. : CSC-RT0261
Host Cell: 293T Target Gene: COA4
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0261 |
| Description | 293T-COA4 (-/-) is a stable cell line with a homozygous knockout of human COA4 |
| Target Gene | COA4 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The exceptional usability of COA4 Knockout Cell Line-293T has made the gene editing process seamless and efficient, eliminating potential technical hurdles and ensuring a smooth research workflow.
COA4 Knockout Cell Line-293T's reliable performance has been particularly valuable in generating consistent results across various experiments, allowing me to draw robust conclusions and build upon previous findings with confidence.
I have witnessed the impact of COA4 Knockout Cell Line-293T not only on my own research but also on the broader scientific community, where its effectiveness has contributed to advancements in genetic research across diverse fields.
The remarkable precision of COA4 Knockout Cell Line-293T has allowed me to explore the intricacies of gene function with unmatched accuracy, uncovering novel insights and opening doors to novel research avenues.
As I continue to use COA4 Knockout Cell Line-293T, I am continually impressed by its versatility, enabling me to investigate gene functions in different cellular contexts and organisms, broadening the scope of my research projects.
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