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Cat.No. : CSC-RT0145
Host Cell: 293T Target Gene: CELF1
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0145 |
| Description | 293T-CELF1 (-/-) is a stable cell line with a homozygous knockout of human CELF1 |
| Background | Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. This gene may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase (DMPK) gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Target Gene | CELF1 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
CELF1 Knockout Cell Line-293T has been a game-changer in my research, providing a reliable and efficient method for precise gene editing.
United States
08/23/2020
I am immediately afterwards impressed by the high knockout efficiency of CELF1 Knockout Cell Line-293T, allowing me to achieve targeted gene modifications with ease.
United States
05/12/2021
The fellow-friendly protocol of CELF1 Knockout Cell Line-293T has simplified the experimental process, making it accessible to researchers with varying levels of expertise.
United States
08/15/2021
The Consistent and reproducible findings obtained using CELF1 Knockout Cell Line-293T have provided a solid foundation for further experimentation and data analysis.
Germany
10/09/2021
I am grateful for the exceptional performance and quality of CELF1 Knockout Cell Line-293T, which has significantly contributed to the success of my research projects.
United States
01/27/2022
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