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Cat.No. : CSC-RT0245
Host Cell: 293T Target Gene: CAP1
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0245 |
| Description | 293T-CAP1 (-/-) is a stable cell line with a homozygous knockout of human CAP1 |
| Background | The protein encoded by this gene is related to the S. cerevisiae CAP protein, which is involved in the cyclic AMP pathway. The human protein is able to interact with other molecules of the same protein, as well as with CAP2 and actin. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2016] |
| Target Gene | CAP1 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
CAP1 Knockout Cell Line-293T has proven to be an invaluable tool in my genetic research, enabling precise and targeted gene modifications with remarkable efficiency.
Germany
10/09/2021
I am impressed by the high success rate of gene knockouts achieved with CAP1 Knockout Cell Line-293T, allowing me to confidently study the functions of specific genes in various biological processes.
United States
01/27/2022
The easy to do protocol and straightforward workflow of CAP1 Knockout Cell Line-293T have simplified the experimental process, making it accessible to researchers with varying levels of expertise.
United States
07/19/2022
The consistent and reproducible results obtained using CAP1 Knockout Cell Line-293T have provided a solid foundation for further investigation and data analysis, strengthening the reliability of my research outcomes.
France
11/03/2022
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