Cat.No. : CSC-RT0223
Host Cell: 293T Target Gene: CALM3
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0223 |
| Description | 293T-CALM3 (-/-) is a stable cell line with a homozygous knockout of human CALM3 |
| Background | This gene encodes a member of a family of proteins that binds calcium and functions as a enzymatic co-factor. Activity of this protein is important in the regulation of the cell cycle and cytokinesis. Multiple alternatively spliced transcript variants have been observed at this gene. [provided by RefSeq, Aug 2016] |
| Target Gene | CALM3 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Beyond CALM3 Knockout Cell Line-293T's exceptional performance in gene knockout, the reagent's versatility has inspired me to explore new avenues of research and investigate the roles of different genes across various biological contexts.
I am delighted with the positive impact CALM3 Knockout Cell Line-293T has had on the overall productivity of my lab, enhancing our research capabilities and contributing to a more dynamic scientific environment.
CALM3 Knockout Cell Line-293T's compatibility with my lab's existing equipment and workflows has allowed for seamless integration, making it a seamless addition to our research toolkit.
As a testament to its effectiveness, I have successfully published multiple research papers with data obtained using CALM3 Knockout Cell Line-293T, contributing to the advancement of scientific knowledge.
I extend my gratitude to the manufacturer for developing such a remarkable gene knockout reagent, which has undoubtedly elevated the quality and significance of my research endeavors.
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