Cat.No. : CSC-RT0181
Host Cell: 293T Target Gene: ATOX1
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0181 |
| Description | 293T-ATOX1 (-/-) is a stable cell line with a homozygous knockout of human ATOX1 |
| Background | This gene encodes a copper chaperone that plays a role in copper homeostasis by binding and transporting cytosolic copper to ATPase proteins in the trans-Golgi network for later incorporation to the ceruloplasmin. This protein also functions as an antioxidant against superoxide and hydrogen peroxide, and therefore, may play a significant role in cancer carcinogenesis. Because of its cytogenetic location, this gene represents a candidate gene for 5q-syndrome. [provided by RefSeq, Jul 2008] |
| Target Gene | ATOX1 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
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I am truly impressed by the effectiveness and reliability of ATOX1 Knockout Cell Line-293T. It delivered consistent and reproducible results, allowing me to draw confident conclusions from my experiments.
ATOX1 Knockout Cell Line-293T's high specificity in targeting the desired gene greatly minimized off-target effects, ensuring the validity of my findings.
Not only did ATOX1 Knockout Cell Line-293T save me valuable time, but it also exhibited a high success rate, surpassing my expectations.
The exceptional customer service provided by the company, with prompt responses to my inquiries and concerns, further enhanced my experience and trust in their product.
I wholeheartedly recommend ATOX1 Knockout Cell Line-293T to fellow researchers who seek a powerful tool for precise gene manipulation and knockout studies.
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