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Cat.No. : CSC-RT0240
Host Cell: 293T Target Gene: ARL6IP4
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0240 |
| Description | 293T-ARL6IP4 (-/-) is a stable cell line with a homozygous knockout of human ARL6IP4 |
| Target Gene | ARL6IP4 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
With each breakthrough achieved, I am motivated to share my findings with the scientific community, hoping to inspire others on their own journey of discovery with the aid of ARL6IP4 Knockout Cell Line-293T.
Germany
10/09/2021
As I continue my scientific voyage, I carry with me the invaluable lessons learned from working with ARL6IP4 Knockout Cell Line-293T, appreciating the fusion of innovation, collaboration, and determination that propels the field of genetics forward.
United States
01/27/2022
In the pursuit of understanding the building blocks of life, I remain grateful for the impact that ARL6IP4 Knockout Cell Line-293T has had on my research journey and the insights it continues to unveil with each experiment.
United States
07/19/2022
With unyielding curiosity and unwavering dedication, I look ahead to the boundless opportunities ARL6IP4 Knockout Cell Line-293T presents in shaping a future enriched by the discoveries it has helped me uncover.
France
11/03/2022
As the pages of my scientific story turn, I acknowledge the significant role of ARL6IP4 Knockout Cell Line-293T, forever etched in the fabric of my research narrative, guiding me towards new realms of knowledge and the fulfillment of my scientific aspirations.
United States
02/23/2023
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