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Cat.No. : CSC-RT0221
Host Cell: 293T Target Gene: ARF3
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0221 |
| Description | 293T-ARF3 (-/-) is a stable cell line with a homozygous knockout of human ARF3 |
| Target Gene | ARF3 |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
ARF3 Knockout Cell Line-293T has ignited a passion for genetic research within me, fuelling my determination to contribute meaningfully to the scientific community.
France
04/11/2023
As I delve deeper into the complexities of gene function, ARF3 Knockout Cell Line-293T's reliability has instilled a sense of confidence in me, bolstering my commitment to unraveling the mysteries of genetics.
United States
05/09/2023
The continuous support and resources provided by the manufacturer have been instrumental in keeping my research on track and pushing the boundaries of my scientific exploration.
United States
08/23/2020
With each successful gene knockout, I am reminded of the immense potential ARF3 Knockout Cell Line-293T holds, driving me to explore novel avenues of inquiry and seek answers to even more challenging questions.
United States
05/12/2021
ARF3 Knockout Cell Line-293T has been a transformative force in my research journey, empowering me to make meaningful contributions to the ever-evolving field of genetics.
United States
08/15/2021
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