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Cat.No. : CSC-RT0244
Host Cell: 293T Target Gene: ADAR
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0244 |
| Description | 293T-ADAR (-/-) is a stable cell line with a homozygous knockout of human ADAR |
| Background | This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010] |
| Target Gene | ADAR |
| Host Cell | 293T |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
As I delve deeper into the mysteries of genetics, I am excited to see how ADAR Knockout Cell Line-293T will continue to unveil new insights, igniting fresh sparks of curiosity and wonder.
United States
10/09/2021
The journey of scientific discovery is akin to a labyrinth, with ADAR Knockout Cell Line-293T acting as a guiding compass, pointing me towards exciting avenues of exploration that lead to unforeseen revelations.
United States
01/27/2022
From the smallest cell to the vast expanse of the genome, ADAR Knockout Cell Line-293T has enabled me to appreciate the intricacies of life's blueprint and how delicate alterations can yield profound consequences.
United States
07/19/2022
Just as a brushstroke contributes to a masterpiece, ADAR Knockout Cell Line-293T has added its brilliance to my scientific canvas, creating a vivid portrait of genetic knowledge and inviting others to pursue understanding.
United States
11/03/2022
With heartfelt appreciation, I embrace the past, savor the present, and eagerly anticipate the future, knowing that ADAR Knockout Cell Line-293T will continue to shape my scientific journey, unraveling the untold stories etched within the DNA of life.
United States
02/23/2023
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