Wheat CRISPR/Cas9 Genome Editing Kit (Basta)

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The Wheat CRISPR/Cas9 Genome Editing Kit is a highly effective and universal gene editing kit. It is intended for Agrobacterium-mediated plant transformation. The CRISPR construct contains a sgRNA expression cassette driven by TaU6 promoter, and a codon optimized Cas9 expression cassette driven by ubiquitin promoter. The Wheat CRISPR/Cas9 Genome Editing Kit is applicable to modify genes in almost all kinds of monocots, especially in wheat.

The Wheat CRISPR/Cas9 Genome Editing Kit is applicable to modify genes in almost all kinds of monocots, especially in wheat.

Cas9 expression cassette and sgRNA expression cassette are combined into one vector which offers a higher transformation efficiency.

•Two functional components are combined into one vector which offers a higher transfection efficiency.
•High expression level of Cas9 protein in monocots by using maize ubiquitin promoter and monocot codon optimized-Cas9.
•High activity of sgRNA in rice by using TaU6 (wheat U6) promoter.
•Mutiplex genome editing

Kanamycin

Basta