Wheat CRISPR/Cas9 Genome Editing Kit (Basta)

Name: Wheat CRISPR/Cas9 Genome Editing Kit (Basta)
SKU: CC-11
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CC-11

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Product Information

Cat. No.	CC-11
Description	The Wheat CRISPR/Cas9 Genome Editing Kit is a highly effective and universal gene editing kit. It is intended for Agrobacterium-mediated plant transformation. The CRISPR construct contains a sgRNA expression cassette driven by TaU6 promoter, and a codon optimized Cas9 expression cassette driven by ubiquitin promoter. The Wheat CRISPR/Cas9 Genome Editing Kit is applicable to modify genes in almost all kinds of monocots, especially in wheat.
Efficiency	Cas9 expression cassette and sgRNA expression cassette are combined into one vector which offers a higher transformation efficiency.
Features	Two functional components are combined into one vector which offers a higher transfection efficiency. High expression level of Cas9 protein in monocots by using maize ubiquitin promoter and monocot codon optimized-Cas9. High activity of sgRNA in rice by using TaU6 (wheat U6) promoter. Mutiplex genome editing
Applications	The Wheat CRISPR/Cas9 Genome Editing Kit is applicable to modify genes in almost all kinds of monocots, especially in wheat.
Resistance	Kanamycin
Selection Marker	Basta

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How to improve the genome editing specificity in wheat?

A: Current findings indicate that targeted editing of genes in polyploid plant genomes can be accomplished via the CRISPR/Cas9 system where a combination of in silico off-target proofing and NGS can be used for the improvement of genome editing specificity.

What are the applications of CRISPR-Cas9 technology in wheat gene editing?

A: CRISPR-Cas9 technology has since been used for targeted knockout of agronomically and scientifically important genes. Traits have been modified using CRISPR-Cas9 which include: lower gluten grain content, increased grain weight, meiotic crossover, reduced preharvest sprouting, and for haploid plant induction.

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Customer Reviews

Great Quality

Excellent quality and exactly as advertised!

United States

2023-01-23

High Transfection Efficiency

Very satisfied with this kit - has a high transformation efficiency.

United States

2022-12-22

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Wheat CRISPR/Cas9 Genome Editing Kit (Basta)

Product Information

Q & A

Customer Reviews

How to improve the genome editing specificity in wheat?

What are the applications of CRISPR-Cas9 technology in wheat gene editing?

Great Quality

High Transfection Efficiency

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