Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : PDPS-AR150
| Cat. No. | PDPS-AR150 |
| Description | Creative Biogene's RotaVirus (Group B) Real Time RT-PCR Kit provides a ready-to-use system for the detection of RotaVirus (Group B) using real-time RT-PCR (Reverse Transcription Polymerase Chain Reaction). |
| Species | virus |
| Features |
• High specificity • High accuracy • Fast & Simple & Efficient |
| Applications | Research Use |
| Nucleic Acid Type | RNA |
| Instrument |
• LightCycler 1.0, LightCycler 2.0 • PE5700, MJ-Opticon & other single color systems • ABI7000, ABI7300, ABI7500, ABI7900, ABI StepOne, StepOne plus, MJ-Opticon2, MJ-chromo4, MX3000P, MX3005P, Smart Cycler II, Rotor-Gene 6000, LightCycler 480, CFX248, Lif96, Slan 96, iCycler iQ4, iCycler iQ5 & other multi-color systems |
| Note | For Research Use Only. Not for use in diagnostic procedures. |
| Storage |
• Store at -20°C • Repeated thawing and freezing (>155x) should be avoided • Cool all reagents during the working steps |
A: To ensure that you get the desired results, first verify that your primers are working, that the template is not degraded, and that the template purity is appropriate, and also determine the best reaction system and reaction conditions for your experiment.
A: First of all, it is important to collect samples in a targeted manner for the purpose of your experiment. The collected samples should preferably be divided into several small portions in lyophilization tubes according to the amount of each experiment and stored at -80°C to avoid repeated freezing. For the extracted sample RNA, it is more important to prevent repeated freezing and thawing and keep the sample fresh.
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I obtained reliable results quickly by using the RotaVirus (Group B) Real Time RT-PCR Kit. According to the instructions, I amplified the control gene and the target gene in the same reaction, rather than separate reactions. This operation minimizes processing errors and improves the reliability of gene quantification.
I am very pleased that my RotaVirus assay went very well with Creative Biogene's RotaVirus (Group B) Real Time RT-PCR Kit. In terms of performance, the kit works very consistently. There is very little error between batches.
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