Human TRIM21 Knockout Cell Line-Hela

Name: Human TRIM21 Knockout Cell Line-Hela
SKU: CSC-RT2817
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2817

Target Gene : TRIM21 Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2817
Description	This cell is a stable cell line with a homozygous knockout of human TRIM21 using CRISPR/Cas9.
Target Gene	TRIM21
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TRIM21 tripartite motif containing 21 [ Homo sapiens ]
Gene Symbol	TRIM21
Synonyms	SSA; RO52; SSA1; RNF81
Gene Description	tripartite motif-containing 21
Gene ID	6737
Uni Prot ID	P19474
m RNA Refseq	NM_003141.3
Protein Refseq	NP_003132.2
Chromosome Location	11p15.5
Function	DNA binding; RNA binding; protein binding; ubiquitin-protein ligase activity; zinc ion binding;
Pathway	Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination & Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem; Systemic lupus erythematosus, organism-specific biosystem;
MIM	109092

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