Human PLAA KO Cell Line-HeLa

Name: Human PLAA KO Cell Line-HeLa
SKU: CSC-RT2993
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2993

Target Gene : PLAA Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2993
Description	This cell is a stable cell line with a homozygous knockout of human PLAA using CRISPR/Cas9
Target Gene	PLAA
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	PLAA phospholipase A2-activating protein [ Homo sapiens ]
Gene Symbol	PLAA
Synonyms	DOA1; PLAP; PLA2P
Gene Description	phospholipase A2-activating protein
Gene ID	9373
Uni Prot ID	Q9Y263
m RNA Refseq	NM_001031689.2
Protein Refseq	NP_001026859.1
Chromosome Location	9p21
Function	phospholipase A2 activator activity; protein binding;
Pathway	Protein processing in endoplasmic reticulum, organism-specific biosystem; Protein processing in endoplasmic reticulum, conserved biosystem;
MIM	603873

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