Human HCCS KO Cell Line-HEK293T

Name: Human HCCS KO Cell Line-HEK293T
SKU: CSC-RT2922
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2922

Target Gene : HCCS Host Cell : HEK293T

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2922
Description	This cell is a stable cell line with a homozygous knockout of human HCCS using CRISPR/Cas9
Target Gene	HCCS
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	HCCS holocytochrome c synthase [ Homo sapiens ]
Gene Symbol	HCCS
Synonyms	HCCS; holocytochrome c synthase; holocytochrome c synthase (cytochrome c heme lyase); cytochrome c-type heme lyase; CCHL; cytochrome c heme lyase; cytochrome c heme-lyase; holocytochrome c-type synthase; MCOPS7; DKFZp779I1858;
GeneID	3052
Uni ProtID	P53701
mRNA Refseq	BC001691
Chromosome Location	Xp22
Function	holocytochrome-c synthase activity; lyase activity; metal ion binding;
Pathway	Porphyrin and chlorophyll metabolism, organism-specific biosystem; Porphyrin and chlorophyll metabolism, conserved biosystem;
MIM	300056

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