Human GNS KO Cell Line-Hela

Name: Human GNS KO Cell Line-Hela
SKU: CSC-RT2899
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2899

Target Gene : GNS Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2899
Description	This cell is a stable cell line with a homozygous knockout of human GNS using CRISPR/Cas9.
Target Gene	GNS
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	GNS glucosamine (N-acetyl)-6-sulfatase [ Homo sapiens ]
Gene Symbol	GNS
Synonyms	GNS; glucosamine (N-acetyl)-6-sulfatase; N-acetylglucosamine-6-sulfatase; N acetylglucosamine 6 sulfatase; Sanfilippo disease IIID; glucosamine-6-sulfatase; G6S; MGC21274;
GeneID	2799
Uni ProtID	P15586
mRNA Refseq	BC012482
Chromosome Location	12q14
Function	N-acetylglucosamine-6-sulfatase activity; glycosaminoglycan binding; hydrolase activity; metal ion binding; protein binding; sulfate binding; sulfuric ester hydrolase activity;
Pathway	Clathrin derived vesicle budding, organism-specific biosystem; Glycosaminoglycan degradation, organism-specific biosystem; Glycosaminoglycan degradation, conserved biosystem; Heparan sulfate degradation, organism-specific biosystem; Heparan sulfate degradation, conserved biosystem; Keratan sulfate degradation, organism-specific biosystem; Keratan sulfate degradation, conserved biosystem;
MIM	607664

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