Human GNAI2 KO Cell Line-HeLa

Name: Human GNAI2 KO Cell Line-HeLa
SKU: CSC-RT2977
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2977

Target Gene : GNAI2 Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2977
Description	This cell is a stable cell line with a homozygous knockout of human GNAI2 using CRISPR/Cas9
Target Gene	GNAI2
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	GNAI2 guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 [ Homo sapiens ]
Gene Symbol	GNAI2
Synonyms	GIP; GNAI2B; H_LUCA15.1; H_LUCA16.1
Gene Description	guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
GeneID	2771
Uni ProtID	B3KP24
mRNA Refseq	NM_002070.2
Protein Refseq	NP_002061.1
Chromosome Location	3p21.31
Function	G-protein beta/gamma-subunit complex binding; G-protein coupled receptor binding; GTP binding; GTPase activity; metal ion binding; protein binding; signal transducer activity;
Pathway	ADP signalling through P2Y purinoceptor 12, organism-specific biosystem; Activation of GABAB receptors, organism-specific biosystem; Adenylate cyclase inhibitory pathway, organism-specific biosystem; Alcoholism, organism-specific biosystem; Alcoholism, conserved biosystem; Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem;
MIM	139360

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